The shotgunator!

This is a simple script that "shotgun sequences" paragraphs of text, by producing "reads" of a configurable length, mutation rate, and coverage. I use it to demonstrate shotgun sequencing and de novo assembly to students.


Enter some text to sequence (leave blank for Tale of Two Cities):

Separate paragraphs by a blank line.

Read length:

Mutation rate (# of mutations in 1000 bp):


Sort results?

Paired ends? If yes, insert size?

Note, the source code is available on github.

C. Titus Brown,